Chimeric coronavirus-like particles carrying severe acute respiratory syndrome coronavirus (SCoV) S protein protect mice against challenge with SCoV.
Identifieur interne : 003147 ( Main/Exploration ); précédent : 003146; suivant : 003148Chimeric coronavirus-like particles carrying severe acute respiratory syndrome coronavirus (SCoV) S protein protect mice against challenge with SCoV.
Auteurs : Kumari G. Lokugamage [États-Unis] ; Naoko Yoshikawa-Iwata ; Naoto Ito ; Douglas M. Watts ; Philip R. Wyde ; Nan Wang ; Patrick Newman ; Chien-Te Kent Tseng ; C J Peters ; Shinji MakinoSource :
- Vaccine [ 0264-410X ] ; 2008.
Descripteurs français
- KwdFr :
- Alignement de séquences, Animaux, Anticorps antiviraux (immunologie), Anticorps antiviraux (sang), Données de séquences moléculaires, Femelle, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (métabolisme), Humains, Injections musculaires, Lignée cellulaire, Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (métabolisme), Protéines de la matrice virale (métabolisme), Souris, Souris de lignée BALB C, Spécificité des anticorps, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (sang), Syndrome respiratoire aigu sévère (virologie), Séquence d'acides aminés, Tests de neutralisation, Vaccination, Vaccins antiviraux (administration et posologie), Virus du SRAS (immunologie), Virus du SRAS (métabolisme), Virus recombinants (immunologie), Virus recombinants (métabolisme).
- MESH :
- administration et posologie : Vaccins antiviraux.
- génétique : Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- immunologie : Anticorps antiviraux, Virus du SRAS, Virus recombinants.
- métabolisme : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines de la matrice virale, Virus du SRAS, Virus recombinants.
- sang : Anticorps antiviraux, Syndrome respiratoire aigu sévère.
- virologie : Syndrome respiratoire aigu sévère.
- Alignement de séquences, Animaux, Données de séquences moléculaires, Femelle, Glycoprotéine de spicule des coronavirus, Humains, Injections musculaires, Lignée cellulaire, Souris, Souris de lignée BALB C, Spécificité des anticorps, Syndrome respiratoire aigu sévère, Séquence d'acides aminés, Tests de neutralisation, Vaccination.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Viral (blood), Antibodies, Viral (immunology), Antibody Specificity, Cell Line, Female, Humans, Injections, Intramuscular, Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), Mice, Mice, Inbred BALB C, Molecular Sequence Data, Neutralization Tests, Reassortant Viruses (immunology), Reassortant Viruses (metabolism), SARS Virus (immunology), SARS Virus (metabolism), Sequence Alignment, Severe Acute Respiratory Syndrome (blood), Severe Acute Respiratory Syndrome (prevention & control), Severe Acute Respiratory Syndrome (virology), Spike Glycoprotein, Coronavirus, Vaccination, Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism), Viral Matrix Proteins (metabolism), Viral Vaccines (administration & dosage).
- MESH :
- chemical , administration & dosage : Viral Vaccines.
- chemical , blood : Antibodies, Viral.
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Viral.
- chemical , metabolism : Membrane Glycoproteins, Viral Envelope Proteins, Viral Matrix Proteins.
- blood : Severe Acute Respiratory Syndrome.
- immunology : Reassortant Viruses, SARS Virus.
- metabolism : Reassortant Viruses, SARS Virus.
- prevention & control : Severe Acute Respiratory Syndrome.
- virology : Severe Acute Respiratory Syndrome.
- Amino Acid Sequence, Animals, Antibody Specificity, Cell Line, Female, Humans, Injections, Intramuscular, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Neutralization Tests, Sequence Alignment, Spike Glycoprotein, Coronavirus, Vaccination.
Abstract
We tested the efficacy of coronavirus-like particles (VLPs) for protecting mice against severe acute respiratory syndrome coronavirus (SCoV) infection. Coexpression of SCoV S protein and E, M and N proteins of mouse hepatitis virus in 293T or CHO cells resulted in the efficient production of chimeric VLPs carrying SCoV S protein. Balb/c mice inoculated with a mixture of chimeric VLPs and alum twice at an interval of four weeks were protected from SCoV challenge, as indicated by the absence of infectious virus in the lungs. The same groups of mice had high levels of SCoV-specific neutralizing antibodies, while mice in the negative control groups, which were not immunized with chimeric VLPs, failed to manifest neutralizing antibodies, suggesting that SCoV-specific neutralizing antibodies are important for the suppression of viral replication within the lungs. Despite some differences in the cellular composition of inflammatory infiltrates, we did not observe any overt lung pathology in the chimeric-VLP-treated mice, when compared to the negative control mice. Our results show that chimeric VLP can be an effective vaccine strategy against SCoV infection.
DOI: 10.1016/j.vaccine.2007.11.092
PubMed: 18191004
Affiliations:
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Lokugamage</name><affiliation wicri:level="2"><nlm:affiliation>Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555, United States.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX 77555</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Yoshikawa Iwata, Naoko" sort="Yoshikawa Iwata, Naoko" uniqKey="Yoshikawa Iwata N" first="Naoko" last="Yoshikawa-Iwata">Naoko Yoshikawa-Iwata</name></author><author><name sortKey="Ito, Naoto" sort="Ito, Naoto" uniqKey="Ito N" first="Naoto" last="Ito">Naoto Ito</name></author><author><name sortKey="Watts, Douglas M" sort="Watts, Douglas M" uniqKey="Watts D" first="Douglas M" last="Watts">Douglas M. 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Sequence Data</term><term>Neutralization Tests</term><term>Sequence Alignment</term><term>Spike Glycoprotein, Coronavirus</term><term>Vaccination</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Animaux</term><term>Données de séquences moléculaires</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Injections musculaires</term><term>Lignée cellulaire</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Spécificité des anticorps</term><term>Syndrome respiratoire aigu sévère</term><term>Séquence d'acides aminés</term><term>Tests de neutralisation</term><term>Vaccination</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We tested the efficacy of coronavirus-like particles (VLPs) for protecting mice against severe acute respiratory syndrome coronavirus (SCoV) infection. Coexpression of SCoV S protein and E, M and N proteins of mouse hepatitis virus in 293T or CHO cells resulted in the efficient production of chimeric VLPs carrying SCoV S protein. Balb/c mice inoculated with a mixture of chimeric VLPs and alum twice at an interval of four weeks were protected from SCoV challenge, as indicated by the absence of infectious virus in the lungs. The same groups of mice had high levels of SCoV-specific neutralizing antibodies, while mice in the negative control groups, which were not immunized with chimeric VLPs, failed to manifest neutralizing antibodies, suggesting that SCoV-specific neutralizing antibodies are important for the suppression of viral replication within the lungs. Despite some differences in the cellular composition of inflammatory infiltrates, we did not observe any overt lung pathology in the chimeric-VLP-treated mice, when compared to the negative control mice. Our results show that chimeric VLP can be an effective vaccine strategy against SCoV infection.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Texas</li></region></list><tree><noCountry><name sortKey="Ito, Naoto" sort="Ito, Naoto" uniqKey="Ito N" first="Naoto" last="Ito">Naoto Ito</name><name sortKey="Kent Tseng, Chien Te" sort="Kent Tseng, Chien Te" uniqKey="Kent Tseng C" first="Chien-Te" last="Kent Tseng">Chien-Te Kent Tseng</name><name sortKey="Makino, Shinji" sort="Makino, Shinji" uniqKey="Makino S" first="Shinji" last="Makino">Shinji Makino</name><name sortKey="Newman, Patrick" sort="Newman, Patrick" uniqKey="Newman P" first="Patrick" last="Newman">Patrick Newman</name><name sortKey="Peters, C J" sort="Peters, C J" uniqKey="Peters C" first="C J" last="Peters">C J Peters</name><name sortKey="Wang, Nan" sort="Wang, Nan" uniqKey="Wang N" first="Nan" last="Wang">Nan Wang</name><name sortKey="Watts, Douglas M" sort="Watts, Douglas M" uniqKey="Watts D" first="Douglas M" last="Watts">Douglas M. Watts</name><name sortKey="Wyde, Philip R" sort="Wyde, Philip R" uniqKey="Wyde P" first="Philip R" last="Wyde">Philip R. Wyde</name><name sortKey="Yoshikawa Iwata, Naoko" sort="Yoshikawa Iwata, Naoko" uniqKey="Yoshikawa Iwata N" first="Naoko" last="Yoshikawa-Iwata">Naoko Yoshikawa-Iwata</name></noCountry><country name="États-Unis"><region name="Texas"><name sortKey="Lokugamage, Kumari G" sort="Lokugamage, Kumari G" uniqKey="Lokugamage K" first="Kumari G" last="Lokugamage">Kumari G. Lokugamage</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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